Skin HealthAnti-AgingEstablishedHuman trials

Argireline

Acetyl Hexapeptide-3 (Acetyl Hexapeptide-8)

Overview

Argireline (Acetyl Hexapeptide-3) is a synthetic hexapeptide derived from the N-terminal end of SNAP-25 — the same protein targeted by botulinum toxin — that has become one of the most popular topical anti-aging peptides globally. Often called 'Botox in a bottle' it reduces expression lines by partially inhibiting neuromuscular junction signaling in facial muscles. Multiple clinical trials have demonstrated reductions in wrinkle depth of 10-27% with consistent application. It is widely used in premium cosmeceutical formulations as a non-invasive alternative to injectable neuromodulators.

Mechanism of Action

Argireline mimics the N-terminal sequence of SNAP-25 and competitively interferes with the SNARE complex formation required for acetylcholine vesicle fusion at the neuromuscular junction. By partially blocking SNARE complex assembly Argireline reduces the quantity of acetylcholine released per nerve impulse leading to partial relaxation of facial expression muscles. The effect is concentration-dependent dose-limited and fully reversible distinguishing it from botulinum toxin which causes complete but temporary denervation. The mechanism is topically targeted — systemic absorption is minimal so effects are local to the application area. Clinical studies have confirmed both the mechanism and efficacy with periocular wrinkle depth reductions comparable to lower concentrations of topical retinol.

Dosage Information

Typical Dose

5-10% concentration in topical formulation

Frequency

Twice daily application to targeted areas

Administration

Topical serum or cream

Notes

Most effective on expression lines around eyes and forehead. Higher concentrations (10%) show significantly better results than lower ones. Combine with moisturizer to prevent any drying effect.

Where does Argireline sit?

See how this peptide compares across all 111 peptides in our database.

Evidence Score

0.68

Clinical trials
1.0035%
Literature
0.6030%
Community
0.0020%
Completeness
1.0015%
3 RCTs

Compound Data

Argireline structure

Molecular Formula

C35H62N14O11S

Molecular Weight

887.00 g/mol

IUPAC Name

(4S)-4-acetamido-5-[[(2S)-1-[[(2S)-1-[[(2S)-5-amino-1-[[(2S)-1-[[(2S)-1-amino-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-1,5-dioxopentan-2-yl]amino]-4-methylsulfanyl-1-oxobutan-2-yl]amino]-4-carboxy-1-oxobutan-2-yl]amino]hex-5-enoic acid

PubChem CID

71587772

Potential Side Effects

Generally very well toleratedMild skin irritation at high concentrations (uncommon)Temporary skin tightness (uncommon)

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Quick Facts

Administration
Topical serum or cream
Typical Dose
5-10% concentration in topical formulation
Frequency
Twice daily application to targeted areas
References
0 curated + 14 from PubMed
Clinical Trials
6 registered
Evidence Score
0.7 / 100